Process for making bismuth sodium gluconate and colloidal solution produced thereby



.romr romemu, or woonsnm FEW: You. ABSIGNOB To an nave um o..

7 v cially intended for t e treatment of syphi best suitable acids to make a soluble alkaline Patented M1122; 1933 Y UNITED, STATES me. or mm: rennin-cm. nxwxoax, a conro'aa'nox or NEW,YOBK' 1 j PA 'r[E 1-.O H -E raocnss ma mule mnm'ra sonnm foauoozurn Ann :couomnieommon f v raonucnn rum? i noel-wing. Application ma November 28,1880. Serial110.498,!1'1.

The resent invention relates to improvements in the manufacture and composition of bismuth sodium luconate colloidal espes I have discoveredrby experimenting with several organic acids to obtain a soluble bismuth salt that gluconic acid is one of the bismuthsalt. I p

The following example for the preparation of bismuth sodium gluconate is given in detail:v V

I begin this preparation, using bismuth subnitrate for making bismuth hydroxide. I take bismuth subnitrate 4.41 gms., which is equal to 3 gms. of metallic bismuth and 'dis-' solve this with acid nitric 6 gms. Then. I add aqua double distilled quantity suflicient to, make 368 cc. Ikthen add to this solution sodium hydroxide 50% solution freshly prepared 6.7 5 cc. addin dropbyv drop and stirrin the solution. by roxide'finely powdered and insoluble in water.

Thenext ste is to filter this through wet filter paper an washthe precipitate several times until free from nitrateorfnitric acid.

The filtrate does not contain an soluble bismuth salt. This can be detecte by addinila few drops of sodium hydroxide to the trate, whichshould not produce a cloudiness or white precipitate.

I then put in a beaker 50 cc. of aqua double distilled and-to this add. 6.56 of acid hen I .add sodium gluconic pure anhydrous. I t en dissolve said acid gluconic pure anhydrous by the aid of entle heath hydroxide 50% solution 5 cc. To this mixture I add the pres cipitated bismuth h droxide of the filter 1- 1s-. solved. ThenI bring the solution to aboil for about two minutes. Then I remove the solution from the burner and let it cool to 'the room temperature.

per and heat gent y-until completely Then I adjust the h drogen ion concentration .pH value of su stantially 8.5 adding sodium. hydroxide solution, 1f necessary. After adjusting the Iadd 2% benzyl alcohol as a" local anesthetic. I then add 24 hoursto settle all impurities ere Iimake a bismuth aqua double distilled-quantity suflicient to make 368 cc. Each 1 cc. of this solution contains 0.008 gms. 0f metallicbismuth.

. It is not necessary to add dextrose sucrose or .anyother sugar protective. colloid. to make the solution colloidal or, to avoid recipitation. I let it stand for not less t an ampuls. I then seal and sterilize not'over 100 C. in boiling water. Autoclaving this v ampul will cause. hydrolysis and separation of bismuth hydroxide. A sample examined in ultra-microscope=shows veryfine colloid q particles with a Brownian movement. ,A

chemical test shows 0008 gm. metallic bis-f .muth. i

Theoretically, the proportions. ofthe ingradients areas follows a M Percent e proportions the ingredients (as found by analysis afe f ll H Although .theebismuth sodium gluconate v v I is not a single vcompound, it is roper to continue the use of the name. I bismuth I sodium luconate were a single compound it .woul not be in true colloidal state.

To find out the minimum lethal dose of this preparation a physiological test was carried albino rats. After extensive experimenting, I found that the minimum lethal dose of this solution calculating 1 cc. per 160 lbs.

on by injecting mypreparationto luconate is a true colloidal solution and does not require the use of a protective colloid either to maintain the said state of aggregation or to create the same. In its method of preparation, no ingredient is added to keep the compound stable. I claim: p 1. 'A process of preparing an aqueous colloidal solution of bismuth sodium gluconate, which consists in treating bismuth subnitrate with calculated amounts of nitric acid and sodium hydroxide to produce a bismuth hydroxide and then adding suflicient gluconic acid and sodium hydroxide to give thereto a pH value of substantially 8.5, resulting in the formation of the aqueous colloidal bismuth sodium gluconate. 2. A process of preparing an aqueous colloidal solution of bismuth sodium gluconate, 2 which consists in treating bismuth subnitrate with calculated amounts of nitric acid and sodium hydroxide, filterin and washing the precipitate, and then ad ing the said precipitate to a solution of a calculated 2 amount ofgluconic acid and sodium hydroxide until the precipitate is dissolved and thereafter adding sufficient sodium hydroxide until a pH value of substantially 8.5 is reached. j $0 3. A process of making bismuth sodium gluconate in colloidal solution, which consists in dissolving bismuth subnitrate in calculated amounts of nitric acid, adding distilled water and sodium hydroxide, filtering and washing the precipitate, dissolving gluconic acid and sodium hydroxide in water and adding the precipitate thereto.

4. A therapeutlc product comprising an aqueous-bismuth sodium gluconate in true i 40 colloidal solution. Signed at New York city inthe county of New York and State of New York this 21 day of November, 1930, A. D.

JOHN TORIGIAN. 

